This kit is used for quantitative determination of insulin (INS) in human serum in vitro. The measurement of insulin (INS) antibody can guide the dosage of insulin and choose the dosage form of insulin for type I diabetic patients. In addition, it can explain the reason of low insulin release curve, that is, the decline of islet function or the combination of antibody and insulin. Because the insulin antibody can pass through the placenta, it can cause the fetal insulin content to reduce.